MFE-280-CAS9 cell line

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > Cas9 Stable Cell Lines > MFE-280-CAS9
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus
  • Wild-type Cell Lines-1 MFE-280-CAS9-Wild-type Cell Lines-1
  • MFE-280-CAS9 cell line MFE-280-CAS9-产品图
MFE-280-CAS9 Copy Product Info
Catalog#: YC-C101-Cas9-H
Size: 1*10^6
Culture method: 88%MEM+10%FBS+1%NEAA+1%1%Sodium Pyruvate
  • Wild-type Cell Lines-1 MFE-280-CAS9-Wild-type Cell Lines-1
  • MFE-280-CAS9 cell line MFE-280-CAS9-产品图
MFE-280-CAS9 Copy Product Info
Catalog#: YC-C101-Cas9-H
Size: 1*10^6
Culture method: 88%MEM+10%FBS+1%NEAA+1%1%Sodium Pyruvate

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 88%MEM+10%FBS+1%NEAA+1%1%Sodium Pyruvate
Passage ratio 1:2-1:3 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
88%MEM+10%FBS+1%NEAA+1%1%Sodium Pyruvate
Passage ratio
1:2-1:3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's MFE-280 cell line was constructed on the basis of wild-type MFE-280 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in MFE-280 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
MFE-280-CAS9 Cas9 14.44739628 -1.21080685
MFE-280-CAS9 β-actin 15.65820313
MFE-280 Cas9 34.59645844 18.11198998
MFE-280 β-actin 16.48446846
Cutting Efficiency Validation
MFE-280-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the MFE-280-CAS9 stable cell pool which is electroporated by ARID2 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:MFE-280
STR Info (Cell bank)
Cell Line:MFE-280
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 10121012
D7S820 10101010
D16S539 12121212
VWA 17171717
TH01 7777
AMEL XXXX
TPOX 910910
CSF1PO 912912
D12S391 2222
FGA 2124
D2S1338 1920
D21S11 3030
D18S51 1318
D8S1179 1113
D3S1358 1818
D6S1043 1416
PENTAE 713
D19S433 1316
PENTAD 99
D1S1656 1516
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Related Popular Products

KO Cell Lines

  • ATRX Knockout cell line (MFE-280)

    ATRX Knockout cell line (MFE-280)

    Organism:
    Gene Name: ATRX
    Gene ID: 546
    Size: 1*10^6
    Catalog#: YKO-H1426
    YKO-H1426(MFE-280)
  • ARID1A Knockout cell line (MFE-280)

    ARID1A Knockout cell line (MFE-280)

    Organism:
    Gene Name: ARID1A
    Gene ID: 8289
    Size: 1*10^6
    Catalog#: YKO-H1425
    YKO-H1425(MFE-280)
  • VHL Knockout cell line (MFE-280)

    VHL Knockout cell line (MFE-280)

    Organism:
    Gene Name: VHL
    Gene ID: 7428
    Size: 1*10^6
    Catalog#: YKO-H1424
    YKO-H1424(MFE-280)

Luciferase Stable Cell Lines

Wild-type Cell Lines

Related Popular Products

KO Cell Lines

  • ATRX Knockout cell line (MFE-280)

    ATRX Knockout cell line (MFE-280)

    Organism:
    Gene Name: ATRX
    Gene ID: 546
    Size: 1*10^6
    Catalog#: YKO-H1426
  • ARID1A Knockout cell line (MFE-280)

    ARID1A Knockout cell line (MFE-280)

    Organism:
    Gene Name: ARID1A
    Gene ID: 8289
    Size: 1*10^6
    Catalog#: YKO-H1425
  • VHL Knockout cell line (MFE-280)

    VHL Knockout cell line (MFE-280)

    Organism:
    Gene Name: VHL
    Gene ID: 7428
    Size: 1*10^6
    Catalog#: YKO-H1424

Luciferase Stable Cell Lines

Wild-type Cell Lines

  • MFE-280 cell line

    Human endometrial adenocarcinoma Cell Line(MFE-280)

    Price (USD): -
    Catalog#: YC-C101
    Size: 1*10^6
    Instruction: Human endometrial adenocarcinoma Cell Line(MFE-280)

×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now
Contact us