RAW 264.7-CAS9 cell line

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location： Home > Products > Cas9 Stable Cell Lines > RAW 264.7-CAS9

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RAW 264.7-CAS9 Order

RAW 264.7-CAS9 cell line
Catalog#
YC-C020-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture Medium-C
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
RAW 264.7-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
RAW 264.7-CAS9
Morphology
monocyte/macrophage,adherent
Passage ratio
1:2-1:3
Species
Mouse
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the RAW 264.7 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a RAW 264.7-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
RAW 264.7-CAS9 Cas9 20.70931435 8.58496571
RAW 264.7-CAS9 h-actin-B 12.12434864
RAW 264.7 Cas9 34.13895035 21.32848454
RAW 264.7 h-actin-B 12.81046581
Cutting Efficiency Validation
RAW 264.7-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the RAW 264.7-CAS9 stable cell pool which is electroporated by Vav3 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03

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