RAW 264.7-CAS9

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RAW 264.7-CAS9

RAW 264.7-CAS9 Order


Price (USD)-

Size 1*10^6


RAW 264.7 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Cell Info

Catalog YC-C020-Cas9-H
Cell line RAW 264.7-CAS9 Cell Type Mouse Macrophage Cell Line
Morphology monocyte/macrophage, adherent Passage ratio 1:2-1:3
Culture method 90%DMEM+10%FBS
Cryopreservation solution 50%DMEM+40% FBS+10%DMSO
Special Note Cells need to be passaged daily, cannot be digested with trypsin. Cells need to be digested by gently pipetting into single cells.

Product Validation Data

Sample NameTarget NameCт MeanΔCт
RAW 264.7-CAS9Cas920.709314358.58496571
RAW 264.7-CAS9h-actin-B12.12434864
RAW 264.7Cas934.1389503521.32848454
RAW 264.7h-actin-B12.81046581
Cutting Efficiency Validation
Note: The above figure shows the sequencing peaks of the RAW 264.7-CAS9 stable cell pool which is electroporated by Vav3 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

  • (1)Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • (2)High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • (3)High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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