U251MG-CAS9 cell line

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U251MG-CAS9 cell line

U251MG-CAS9 cell line U251MG-CAS9-产品图

U251MG-CAS9 Copy Product Info

Catalog#： YC-D015-Cas9-H

Size： 1*10^6

Culture method： 90% DMEM+10%FBS

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STR Report

Datasheet (Use Instruction)

U251MG-CAS9 cell line

U251MG-CAS9 Copy Product Info

Catalog#： YC-D015-Cas9-H

Size： 1*10^6

Culture method： 90% DMEM+10%FBS

Order

STR Report

Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category	Cas9 Stable Cell Lines	Mycoplasma test	negative
Cell Morphology	Epithelial-like, adherent	Culture method	90% DMEM+10%FBS
Passage ratio	1:2~1:4	Species	Human
Expression gene	Cas9	Resistance gene	hygro

Product category

Cas9 Stable Cell Lines

Mycoplasma test

negative

Cell Morphology

Epithelial-like, adherent

Culture method

90% DMEM+10%FBS

Passage ratio

1:2~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's U251MG cell line was constructed on the basis of wild-type U251MG cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in U251MG cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR

Sample Name	Target Name	Cт Mean	ΔCт
U251MG-CAS9	Cas9	14.25889301	-1.58300591
U251MG-CAS9	β-actin	15.84189892	-1.58300591
U251MG	Cas9	35.44923401	19.64437771
U251MG	β-actin	15.80485630	19.64437771

Cutting Efficiency Validation

U251MG-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the U251MG-CAS9 stable cell pool which is electroporated by SLC30A9 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Download STR Report

Loci	STR Info (Sample Cell) Sample Cell Line:U251MG			STR Info (Cell bank) Cell Line:U-251MG
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	10	11		10	11
D7S820	10	12		10	12
D16S539	12	12		12	12
VWA	16	18		16	18
TH01	9.3	9.3		9.3	9.3
AMEL	X	X		X	Y
TPOX	8	8		8	8
CSF1PO	11	12		11	12
D12S391	17	22
FGA	21	25
D2S1338	24	24
D21S11	29	29
D18S51	13	13
D8S1179	13	15
D3S1358	16	16
D6S1043	12	12
PENTAE	7	10
D19S433	13	15
PENTAD	12	12
D1S1656	16	17.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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U251MG-CAS9 cell line

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