VERO C1008 (E6)-CAS9 cell line

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VERO C1008 (E6)
VERO C1008 (E6)-CAS9 cell line

VERO C1008 (E6) VERO C1008 (E6)-CAS9-VERO C1008 (E6)

VERO C1008 (E6)-CAS9 cell line VERO C1008 (E6)-CAS9-产品图

VERO C1008 (E6)-CAS9 Copy Product Info

Catalog#： YC-A003-Cas9-H

Size： 1*10^6

Culture method： 90%DMEM+10% FBS

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Datasheet (Use Instruction)

VERO C1008 (E6)
VERO C1008 (E6)-CAS9 cell line

VERO C1008 (E6)-CAS9 Copy Product Info

Catalog#： YC-A003-Cas9-H

Size： 1*10^6

Culture method： 90%DMEM+10% FBS

Order

Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Product category	Cas9 Stable Cell Lines	Mycoplasma test	negative
Cell Morphology	Epithelial-like, adherent	Culture method	90%DMEM+10% FBS
Passage ratio	1:2~1:4	Species	Green monkey
Expression gene	Cas9	Resistance gene	hygro

Product category

Cas9 Stable Cell Lines

Mycoplasma test

negative

Cell Morphology

Epithelial-like, adherent

Culture method

90%DMEM+10% FBS

Passage ratio

1:2~1:4

Species

Green monkey

Expression gene

Cas9

Resistance gene

hygro

Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's VERO C1008 (E6) cell line was constructed on the basis of wild-type VERO C1008 (E6) cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in VERO C1008 (E6) cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR

Sample Name	Target Name	Cт Mean	ΔCт
VERO C1008 (E6)-CAS9	Cas9	18.50941467	1.70602417
VERO C1008 (E6)-CAS9	β-actin	16.80339050	1.70602417
VERO C1008 (E6)	Cas9	32.85825729	13.93244743
VERO C1008 (E6)	β-actin	18.92580986	13.93244743

Cutting Efficiency Validation

VERO C1008 (E6)-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the VERO C1008 (E6)-CAS9 stable cell pool which is electroporated by GSDME gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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VERO C1008 (E6)-CAS9 cell line

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