VERO C1008 (E6)-CAS9 cell line

EN CN
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location： Home > Products > Cas9 Stable Cell Lines > VERO C1008 (E6)-CAS9

     gRNA Plasmid Bank

     Cell Lines

KO Cell Lines

Wild-type Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cas9 Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Luciferase Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

EGFP Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Stem Cell Lines

     Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

     Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

     Lentivirus

gene KO/PM cell lines
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

VERO C1008 (E6)-CAS9 Order

VERO C1008 (E6)-CAS9 cell line
Catalog#
YC-A003-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-A
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
VERO C1008 (E6)-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
VERO C1008 (E6)-CAS9
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Green monkey
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the VERO C1008 (E6) cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a VERO C1008 (E6)-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
VERO C1008 (E6)-CAS9 Cas9 18.50941467 1.70602417
VERO C1008 (E6)-CAS9 h-actin-B 16.80339050
VERO C1008 (E6) Cas9 32.85825729 13.93244743
VERO C1008 (E6) h-actin-B 18.92580986
Cutting Efficiency Validation
VERO C1008 (E6)-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the VERO C1008 (E6)-CAS9 stable cell pool which is electroporated by NECTIN1 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03

Related Popular Products

KO Cell Lines

  • ATG16L1 Knockout cell line (VERO C1008 (E6))

    ATG16L1 Knockout cell line (VERO C1008 (E6))
      inquiry   More details

    Organism: Green monkey
    Gene Name: ATG16L1
    Gene ID: 103218104
    Size: 1*10^6
    Catalog#: YKO-R058
    YKO-R058(VERO C1008 (E6))
  • STAT1 Knockout cell line (VERO C1008 (E6))

    STAT1 Knockout cell line (VERO C1008 (E6))
      inquiry   More details

    Organism: Green monkey
    Gene Name: STAT1
    Gene ID: 103217529
    Size: -
    Catalog#: YKO-R013
    YKO-R013(VERO C1008 (E6))
  • STAT3 Knockout cell line (VERO C1008 (E6))

    STAT3 Knockout cell line (VERO C1008 (E6))
      inquiry   More details

    Organism: Green monkey
    Gene Name: STAT3
    Gene ID: 103243410
    Size: 1*10^6
    Catalog#: YKO-R045
    YKO-R045(VERO C1008 (E6))

Related Popular Products

KO Cell Lines

  • ATG16L1 Knockout cell line (VERO C1008 (E6))

    ATG16L1 Knockout cell line (VERO C1008 (E6))

    Organism: Green monkey
    Gene Name: ATG16L1
    Gene ID: 103218104
    Size: 1*10^6
    Catalog#: YKO-R058
      inquiry   More details
  • STAT1 Knockout cell line (VERO C1008 (E6))

    STAT1 Knockout cell line (VERO C1008 (E6))

    Organism: Green monkey
    Gene Name: STAT1
    Gene ID: 103217529
    Size: -
    Catalog#: YKO-R013
      inquiry   More details
  • STAT3 Knockout cell line (VERO C1008 (E6))

    STAT3 Knockout cell line (VERO C1008 (E6))

    Organism: Green monkey
    Gene Name: STAT3
    Gene ID: 103243410
    Size: 1*10^6
    Catalog#: YKO-R045
      inquiry   More details

WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
Contact us