ACHN-CAS9
Red cotton OmniCell bank
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Online inquireOverview
The Cas9 nuclease has been stably integrated into the host cell genome via genetic engineering methods, enabling stable, long-term expression of Cas9 protein. Cas9 stable cell lines serve as essential tools for CRISPR gene knockout, CRISPR library screening, and functional genomics studies. Ubigene has developed more than 500 Cas9 stable cell lines covering commonly used cell types. Gene knockout can be rapidly achieved by simply transfecting gRNA. Co-transfection of gRNA with donor DNA further enables gene knock-in or precise point mutations.
The Ubigene RKO-Cas9 cell line was generated using a lentiviral method and selected with antibiotics to achieve stable, high-level Cas9 expression. The RKO-CAS9 cell line has undergone functional editing validation, with results demonstrating that introduction of target gene gRNA can produce efficient DNA cleavage and indels, making it suitable for gene knockout, gRNA efficiency validation, or high-throughput CRISPR library screening in RKO cells.
Detailed Product Information
The ACHN cell line was initiated in November, 1979 from the malignant pleural effusion of a 22-year-old Caucasian male with widely metastatic renal adenocarcinoma (autopsy confirmed). Cells were seeded directly to culture flasks in Eagle's MEM with 10% FBS, then maintained and passaged 150 days in flasks. Cells were then inoculated subcutaneously into nude mice. After 4 weeks, palpable, locally invasive tumors were noted. Both the original cells (ACHN) and those recovered from nude mouse tumors were growth-inhibited by human interferons.
Product Advantages
Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
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Intended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use
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