ACHN-EGFP
Red cotton OmniCell bank
Online inquireOverview
ACHN-EGFP is a cell line stably expressing EGFP protein. This cell line was generated using lentivirus method and enables stable and high-efficiency expression of enhanced green fluorescent protein (EGFP). Unlike enzymatic reporter systems, no additional enzymes or substrates are required for signal detection. The cells emit strong fluorescence under UV or blue light excitation without significant quenching and can be detected via in vitro and in vivo bioluminescence assays.
ACHN-EGFP cell line has been optimized to maintain low passage number, high viability, and good condition. It is suitable for a wide range of applications, including high-throughput drug screening, in vivo fluorescence tracing, stable cell line construction, and serves as a control cell line in lentiviral infection experiments.
Detailed Product Information
The ACHN cell line was initiated in November, 1979 from the malignant pleural effusion of a 22-year-old Caucasian male with widely metastatic renal adenocarcinoma (autopsy confirmed). Cells were seeded directly to culture flasks in Eagle's MEM with 10% FBS, then maintained and passaged 150 days in flasks. Cells were then inoculated subcutaneously into nude mice. After 4 weeks, palpable, locally invasive tumors were noted. Both the original cells (ACHN) and those recovered from nude mouse tumors were growth-inhibited by human interferons.
Product Advantages
Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
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Intended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use
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