AHNAK2 Knockout cell line(A549)

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AHNAK2 Knockout cell line(A549)

AHNAK2 Knockout cell line(A549) Order

Catalog#YKO-H593

Price (USD)-

Size 1*10^6

Instruction

Exclusive Red Cotton system(en.rc-crispr.com)was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Cell Info

Catalog YKO-H593
Cell line AHNAK2 Knockout cell line(A549) Cell Type Human Lung Cancer Cell Line
Morphology Epithelial-like, adherent Passage ratio 1:3~1:5
Culture method 90% F12K+10% FBS
Cryopreservation solution 50%F12K+40%FBS+10%DMSO
Special Note

STR Validation

Gene Information

Official symbol
AHNAK2
Gene id
113146
Official full symbol
AHNAK nucleoprotein 2
Also known as
C14orf78
This gene encodes a large nucleoprotein. The encoded protein has a tripartite domain structure with a relatively short N-terminus and a long C-terminus, separated by a large body of repeats. The N-terminal PSD-95/Discs-large/ZO-1 (PDZ)-like domain is thought to function in the formation of stable homodimers. The encoded protein may play a role in calcium signaling by associating with calcium channel proteins. Alternative splicing results in multiple transcript variants.

KO strategy

Knockout region: Exon 2,Exon 3 (based on transcript AHNAK2-201)
Validation: PCR+Sanger Sequencing

Method

Exclusive Red Cotton system(en.rc-crispr.com)was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

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