AIFM2 Knockout cell line(HT-1080)

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AIFM2 Knockout cell line(HT-1080) Order

AIFM2 Knockout cell line(HT-1080)
Catalog#
YKO-H363
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
AIFM2 Knockout cell line(HT-1080)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
AIFM2 Knockout cell line (HT-1080)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:4
Species
Human
Gene Name
AIFM2
Gene ID
84883
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript AIFM2-201)
Validation: PCR+Sanger Sequencing
AIFM2 Knockout cell line(HT-1080) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HT-1080
STR Info (Cell bank)
Cell Line:HT-1080
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 12141214
D7S820 910910
D16S539 912912
VWA 14191419
TH01 6666
AMEL XYXY
TPOX 8888
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AIFM2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT-1080 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AIFM2 gene knockout AIFM2 Knockout cell line (HT-1080) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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