ARHGEF18 Knockout cell line (IPSC)

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ARHGEF18 Knockout cell line (IPSC) Order

ARHGEF18 Knockout cell line (IPSC)
Catalog#
YKO-H985
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ARHGEF18 Knockout cell line (IPSC)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ARHGEF18 Knockout cell line(IPSC-DYR0100)
Cell Morphology
Spherical clone
Passage ratio
1:6-1:8
Species
Human
Gene Name
ARHGEF18
Gene ID
23370
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
EZ-Stem™ Cell Culture Medium
Target region
Knockout region: Exon 11 (based on transcript ARHGEF18-206)
Validation: PCR+Sanger Sequencing
ARHGEF18 Knockout cell line (IPSC) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:IPSC-DYR0100
STR Info (Cell bank)
Cell Line:ATCC- DYR0100 hiPSC
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11131113
D7S820 910910
D16S539 12121212
VWA 14191419
TH01 9.39.39.39.3
AMEL XYXY
TPOX 811811
CSF1PO 711711
D12S391 1819.3
FGA 2122
D2S1338 1719
D21S11 3032.2
D18S51 1013
D8S1179 1415
D3S1358 1517
D6S1043 1213
PENTAE 77
D19S433 1415
PENTAD 1213
D1S1656 1217.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ARHGEF18 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the IPSC-DYR0100 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ARHGEF18 gene knockout ARHGEF18 Knockout cell line(IPSC-DYR0100) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ARHGEF18 Knockout cell line (IPSC)
ARHGEF18 Knockout cell line (IPSC)
Catalog#
YKO-H985
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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