Ahr Knockout cell line (RAW264.7)

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Ahr Knockout cell line (RAW264.7) Order

Ahr Knockout cell line (RAW264.7)
Catalog#
YKO-M166
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Ahr Knockout cell line (RAW264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Ahr Knockout cell line (RAW 264.7)
Morphology
monocyte/macrophage,adherent
Passage ratio
1:2-1:3
Species
Mouse
Gene Name
Ahr
Gene ID
11622
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 3 (based on transcript Ahr-202)
Validation: PCR+Sanger Sequencing
Ahr Knockout cell line (RAW264.7) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:RAW 264.7
STR Info (Cell bank)
Cell Line:RAW 264.7
Allele1Allele2Allele3Allele1Allele2Allele3
4-2 245.46
【22.3】
22.3
5-5 335.54
【14】
14.0
6-4 299.7
【18】
18.0
6-7 334.21
【12】
12.0
9-2 221.01
【15】
15.0
12-1 225.23
【16】
16.0
15-3 200.62
【22.3】
22.3
18-3 159.66
【18】
18.0
X-1 396.51
【24】
24.0
D4S2408
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Ahr gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RAW 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Ahr gene knockout Ahr Knockout cell line (RAW 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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