B3GNT2 Knockout cell line(A375)

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B3GNT2 Knockout cell line(A375) Order

B3GNT2 Knockout cell line(A375)
Catalog#
YKO-H486
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
B3GNT2 Knockout cell line(A375)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
B3GNT2 Knockout cell line (A375)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
B3GNT2
Gene ID
10678
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript B3GNT2-201)
Validation: PCR+Sanger Sequencing
B3GNT2 Knockout cell line(A375) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:A375
STR Info (Cell bank)
Cell Line:A-375 [A375]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11141114
D7S820 9999
D16S539 9999
VWA 16171617
TH01 8888
AMEL XXXX
TPOX 810810
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the B3GNT2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A375 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, B3GNT2 gene knockout B3GNT2 Knockout cell line (A375) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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