BAD Knockout cell line (NCI-H1975)

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location： Home > Products > KO Cell Lines > BAD Knockout cell line (NCI-H1975)

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BAD Knockout cell line (NCI-H1975) Order

BAD Knockout cell line (NCI-H1975)
Catalog#
YKO-H1099
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
BAD Knockout cell line (NCI-H1975)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
BAD Knockout cell line (NCI-H1975)
Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：4
Species
Human
Gene Name
BAD
Gene ID
572
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_E0ZS(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript BAD-201)
Validation: PCR+Sanger Sequencing
BAD Knockout cell line (NCI-H1975) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H1975		 STR Info (Cell bank)
Cell Line:NCI-H1975
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 10131013
D7S820 88811
D16S539 912912
VWA 18181818
TH01 7777
AMEL XXXX
TPOX 811811
CSF1PO 12121212
D12S391 1717
FGA 2124
D2S1338 1717
D21S11 2828
D18S51 1313
D8S1179 1313
D3S1358 1415
D6S1043 1212
PENTAE 1212
D19S433 1515.2
PENTAD 1213
D1S1656 1415
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BAD gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NCI-H1975 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BAD gene knockout BAD Knockout cell line (NCI-H1975) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
BAD Knockout cell line (NCI-H1975)
BAD Knockout cell line (NCI-H1975)
Catalog#
YKO-H1099
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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