BCHE Knockout cell line (A549)

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location： Home > Products > KO Cell Lines > BCHE Knockout cell line (A549)

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BCHE Knockout cell line (A549) Order

BCHE Knockout cell line (A549)
Catalog#
YKO-XN85852
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
BCHE Knockout cell line (A549)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
BCHE Knockout cell line (A549)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
BCHE
Gene ID
590
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% F12K+10% FBS
Target region
Knockout region: Exon 2 (based on transcript BCHE-201)
Validation: PCR+Sanger Sequencing
BCHE Knockout cell line (A549) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BCHE gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BCHE gene knockout BCHE Knockout cell line (A549) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
BCHE Knockout cell line (A549)
BCHE Knockout cell line (A549)
Catalog#
YKO-XN85852
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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