Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation
CRISPR-U™ technology. At the same time, we already provide customers with high quality gene-editing
tools for cell or animal research worldwide.
With 14 years of experience, Ubigene has exclusively innovated and developed 6 product lines,
fullfilling all kinds of needs from researchers. Experiment process simplified, efficiency improved,
achieving our aim of 'Make genome editing easier'!
Knockout region: None (based on transcript None)
Validation: PCR+Sanger Sequencing
Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy
for the CADM1 gene, clone the two sgRNAs into CRISPR-U™ vector,
transfect the vector into the MDCK cell line by electroporation/lentivirus method, use the limited dilution
method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic
acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype.
Upon passing the verification, CADM1 gene knockout CADM1 Knockout cell line (MDCK) will be expanded and cryopreserved.