CASP9 Knockout cell line(HEK293)

location: Home > Products > KO Cell Lines > CASP9 Knockout cell line(HEK293)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

CASP9 Knockout cell line(HEK293) Order

CASP9 Knockout cell line(HEK293)
Catalog#
YKO-H372
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CASP9 Knockout cell line(HEK293)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CASP9 Knockout cell line (HEK293)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
CASP9
Gene ID
842
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 2 (based on transcript CASP9-201)
Validation: PCR+Sanger Sequencing
CASP9 Knockout cell line(HEK293) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HEK293
STR Info (Cell bank)
Cell Line:HEK-293.2sus
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 8888
D13S317 12141214
D7S820 11121112
D16S539 913913
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 11111111
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CASP9 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CASP9 gene knockout CASP9 Knockout cell line (HEK293) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

  • HEK293 cell line

    Human Embryonic Kidney Cell Line(HEK293)

    Catalog#: YC-A008
    Size: 1*10^6
    Instruction: Human Embryonic Kidney Cell Line(HEK293)

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • CASP9 Knockout cell line (A549)

    CASP9 Knockout cell line (A549)

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-H705
    YKO-H705(A549)
  • CASP9 Knockout cell line (HCT 116)

    CASP9 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-HS2836
    YKO-HS2836(HCT 116)
  • CASP9 Knockout cell line(Hela)

    CASP9 Knockout cell line(Hela)

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: -
    Catalog#: YKO-KP110
    YKO-KP110(Hela)

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • CASP9 Knockout cell line (A549)

    CASP9 Knockout cell line (A549)

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-H705
  • CASP9 Knockout cell line (HCT 116)

    CASP9 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: 1*10^6
    Catalog#: YKO-HS2836
  • CASP9 Knockout cell line(Hela)

    CASP9 Knockout cell line(Hela)

    Organism: Human
    Gene Name: CASP9
    Gene ID: 842
    Size: -
    Catalog#: YKO-KP110

Contact us