CCDC80 Knockout cell line (HEK293)

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location: Home > Products > KO Cell Lines > CCDC80 Knockout cell line (HEK293)
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CCDC80 Knockout cell line (HEK293) Order

CCDC80 Knockout cell line (HEK293)
Catalog#
YKO-XN86498
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CCDC80 Knockout cell line (HEK293)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CCDC80 Knockout cell line (HEK293)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
CCDC80
Gene ID
151887
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 2 (based on transcript CCDC80-201)
Validation: PCR+Sanger Sequencing
CCDC80 Knockout cell line (HEK293) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CCDC80 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CCDC80 gene knockout CCDC80 Knockout cell line (HEK293) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CCDC80 Knockout cell line (HEK293)
CCDC80 Knockout cell line (HEK293)
Catalog#
YKO-XN86498
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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KO Cell Lines

  • CCDC80 Knockout cell line (HeLa)

    CCDC80 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: CCDC80
    Gene ID: 151887
    Size: 1*10^6
    Catalog#: YKO-XN86499
  • CCDC80 Knockout cell line (A549)

    CCDC80 Knockout cell line (A549)

    Organism: Human
    Gene Name: CCDC80
    Gene ID: 151887
    Size: 1*10^6
    Catalog#: YKO-XN86497
  • CCDC80 Knockout cell line (Hep G2)

    CCDC80 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: CCDC80
    Gene ID: 151887
    Size: 1*10^6
    Catalog#: YKO-H270

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