COL12A1 Knockout cell line (HCT 116)

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COL12A1 Knockout cell line (HCT 116) Order

COL12A1 Knockout cell line (HCT 116)
Catalog#
YKO-H1043
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
COL12A1 Knockout cell line (HCT 116)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
COL12A1 Knockout cell line (HCT 116)
Species
Human
Gene Name
COL12A1
Gene ID
1303
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 6 (based on transcript COL12A1-201)
Validation: PCR+Sanger Sequencing
COL12A1 Knockout cell line (HCT 116) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the COL12A1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, COL12A1 gene knockout COL12A1 Knockout cell line (HCT 116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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