DTX3L Knockout cell line (HUVEC)

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DTX3L Knockout cell line (HUVEC) Order

DTX3L Knockout cell line (HUVEC)
Catalog#
YKO-KP179
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
DTX3L Knockout cell line (HUVEC)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
DTX3L Knockout cell line (HUVEC)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Gene Name
DTX3L
Gene ID
151636
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
Target region
Knockout region: Exon 2,Exon 3 (based on transcript DTX3L-201)
Validation: PCR+Sanger Sequencing
DTX3L Knockout cell line (HUVEC) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the DTX3L gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HUVEC cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, DTX3L gene knockout DTX3L Knockout cell line (HUVEC) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
DTX3L Knockout cell line (HUVEC)
DTX3L Knockout cell line (HUVEC)
Catalog#
YKO-KP179
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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