FBXW7 Knockout cell line(5637)

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FBXW7 Knockout cell line(5637) Order

FBXW7 Knockout cell line(5637)
Catalog#
YKO-H519
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
FBXW7 Knockout cell line(5637)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
FBXW7 Knockout cell line (5637)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Gene Name
FBXW7
Gene ID
55294
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 4 (based on transcript FBXW7-201)
Validation: PCR+Sanger Sequencing
FBXW7 Knockout cell line(5637) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:5637
STR Info (Cell bank)
Cell Line:5637
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11111111
D7S820 10111011
D16S539 9999
VWA 18181818
TH01 7979
AMEL XXXY
TPOX 8889
CSF1PO 11111111
D12S391 2020
FGA 2222
D2S1338 2525
D21S11 3636
D18S51 1618
D8S1179 1010
D3S1358 1517
D6S1043 1620
PENTAE 1012
D19S433 1315
PENTAD 1111
D1S1656 1515
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FBXW7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 5637 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FBXW7 gene knockout FBXW7 Knockout cell line (5637) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
FBXW7 Knockout cell line(5637)
FBXW7 Knockout cell line(5637)
Catalog#
YKO-H519
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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