FBXW7 Knockout cell line(SW480)

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FBXW7 Knockout cell line(SW480) Order

FBXW7 Knockout cell line(SW480)
Catalog#
YKO-H499
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
FBXW7 Knockout cell line(SW480)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
FBXW7 Knockout cell line (SW480)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
FBXW7
Gene ID
55294
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 4 (based on transcript FBXW7-201)
Validation: PCR+Sanger Sequencing
FBXW7 Knockout cell line(SW480) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:SW480
STR Info (Cell bank)
Cell Line:SW-480
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 12121212
D7S820 8888
D16S539 13131313
VWA 16161616
TH01 8888
AMEL XXXX
TPOX 11111111
CSF1PO 13141314
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FBXW7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SW480 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FBXW7 gene knockout FBXW7 Knockout cell line (SW480) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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