GLMP Knockout cell line (HeLa)

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GLMP Knockout cell line (HeLa) Order

GLMP Knockout cell line (HeLa)
Catalog#
YKO-H574
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GLMP Knockout cell line (HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GLMP Knockout cell line (HeLa)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
GLMP
Gene ID
112770
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 3 (based on transcript GLMP-201)
Validation: PCR+Sanger Sequencing
GLMP Knockout cell line (HeLa) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GLMP gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GLMP gene knockout GLMP Knockout cell line (HeLa) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GLMP Knockout cell line (HeLa)
GLMP Knockout cell line (HeLa)
Catalog#
YKO-H574
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • GLMP Knockout cell line (HCT 116)

    GLMP Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: GLMP
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    Size: 1*10^6
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    Organism: Human
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