HDGF Knockout cell line(HT-29)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > HDGF Knockout cell line(HT-29)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

HDGF Knockout cell line(HT-29) Order

HDGF Knockout cell line(HT-29)
Catalog#
YKO-H479
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
HDGF Knockout cell line(HT-29)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
HDGF Knockout cell line (HT-29)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
HDGF
Gene ID
3068
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Target region
Knockout region: Exon 3 (based on transcript HDGF-201)
Validation: PCR+Sanger Sequencing
HDGF Knockout cell line(HT-29) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:HT-29
STR Info (Cell bank)
Cell Line:HT-29
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11121112
D7S820 10101010
D16S539 11121112
VWA 17191719
TH01 6969
AMEL XXXX
TPOX 8989
CSF1PO 11121112
D12S391 18.321
FGA 2022
D2S1338 1923
D21S11 2930
D18S51 1313
D8S1179 1010
D3S1358 1517
D6S1043 1214
PENTAE 1416
D19S433 1414.2
PENTAD 1113
D1S1656 1516
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the HDGF gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT-29 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, HDGF gene knockout HDGF Knockout cell line (HT-29) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
HDGF Knockout cell line(HT-29)
HDGF Knockout cell line(HT-29)
Catalog#
YKO-H479
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • HDGF Knockout cell line (HeLa)

    HDGF Knockout cell line (HeLa)

    Organism: Human
    Gene Name: HDGF
    Gene ID: 3068
    Size: 1*10^6
    Catalog#: YKO-XN89187
    YKO-XN89187(HeLa)
  • HDGF Knockout cell line (HCT 116)

    HDGF Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: HDGF
    Gene ID: 3068
    Size: 1*10^6
    Catalog#: YKO-XN89186
    YKO-XN89186(HCT 116)
  • HDGF Knockout cell line (HEK293)

    HDGF Knockout cell line (HEK293)

    Organism: Human
    Gene Name: HDGF
    Gene ID: 3068
    Size: 1*10^6
    Catalog#: YKO-XN89185
    YKO-XN89185(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • HDGF Knockout cell line (HeLa)

    HDGF Knockout cell line (HeLa)

    Organism: Human
    Gene Name: HDGF
    Gene ID: 3068
    Size: 1*10^6
    Catalog#: YKO-XN89187
  • HDGF Knockout cell line (HCT 116)

    HDGF Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: HDGF
    Gene ID: 3068
    Size: 1*10^6
    Catalog#: YKO-XN89186
  • HDGF Knockout cell line (HEK293)

    HDGF Knockout cell line (HEK293)

    Organism: Human
    Gene Name: HDGF
    Gene ID: 3068
    Size: 1*10^6
    Catalog#: YKO-XN89185

Contact us