HMGB1 Knockout cell line(LS 174T)

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HMGB1 Knockout cell line(LS 174T) Order

HMGB1 Knockout cell line(LS 174T)
Catalog#
YKO-H913
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
HMGB1 Knockout cell line(LS 174T)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
HMGB1 Knockout cell line(LS 174T)
Morphology
Epithelial-like,adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
HMGB1
Gene ID
3146
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 3 (based on transcript HMGB1-203)
Validation: PCR+Sanger Sequencing
HMGB1 Knockout cell line(LS 174T) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the HMGB1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the LS 174T cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, HMGB1 gene knockout HMGB1 Knockout cell line(LS 174T) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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