HMGB1 Knockout cell line (Hela)

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HMGB1 Knockout cell line (Hela)

HMGB1 Knockout cell line (Hela) Order

Catalog#YKO-H790

Price (USD)-

Size 1*10^6

Instruction

Exclusive Red Cotton system(en.rc-crispr.com)was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

Cell Info

Catalog YKO-H790
Cell line HMGB1 Knockout cell line (Hela) Cell Type Human Cervical Carcinoma Cell Line
Morphology Epithelial-like, adherent Passage ratio 1:3~1:6
Culture method 90%DMEM+10% FBS
Cryopreservation solution 50%DMEM+40% FBS+10%DMSO
Special Note

STR Validation

Gene Information

Official symbol
HMGB1
Gene id
3146
Official full symbol
high mobility group box 1
Also known as
HMG-1, HMG1, HMG3, SBP-1
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

KO strategy

Knockout region: Exon 3 (based on transcript HMGB1-203)
Validation: PCR+Sanger Sequencing

Method

Exclusive Red Cotton system(en.rc-crispr.com)was used to design the sgRNA fragment knockout strategy. Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. Upon passing the final validation, the positive clones were expanded and cryopreserved.

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