ABCA1 Knockout cell line(ARPE-19)

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ABCA1 Knockout cell line(ARPE-19) Order

ABCA1 Knockout cell line(ARPE-19)
Catalog#
YKO-H173
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ABCA1 Knockout cell line(ARPE-19)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ABCA1 Knockout cell line (ARPE-19)
Morphology
Endothelial cells, adherent
Passage ratio
1:3~1:5
Species
Human
Gene Name
ABCA1
Gene ID
19
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM/F12+10% FBS
Target region
Knockout region: Exon 5 (based on transcript ABCA1-202)
Validation: PCR+Sanger Sequencing
ABCA1 Knockout cell line(ARPE-19) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:ARPE-19
STR Info (Cell bank)
Cell Line:ARPE-19
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11121112
D7S820 911911
D16S539 911911
VWA 16191619
TH01 69.369.3
AMEL XYXY
TPOX 911911
CSF1PO 11111111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ABCA1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the ARPE-19 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ABCA1 gene knockout ABCA1 Knockout cell line (ARPE-19) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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