ACSF3 Knockout cell line(AGS)

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ACSF3 Knockout cell line(AGS) Order

ACSF3 Knockout cell line(AGS)
Catalog#
YKO-H184
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ACSF3 Knockout cell line(AGS)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ACSF3 Knockout cell line (AGS)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
ACSF3
Gene ID
197322
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% F12K+10% FBS
Target region
Knockout region: Exon 3 (based on transcript ACSF3-218)
Validation: PCR+Sanger Sequencing
ACSF3 Knockout cell line(AGS) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:AGS
STR Info (Cell bank)
Cell Line:AGS
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 912912
D13S317 12121212
D7S820 10111011
D16S539 11131113
VWA 16171617
TH01 6767
AMEL XXXX
TPOX 11121112
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ACSF3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the AGS cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ACSF3 gene knockout ACSF3 Knockout cell line (AGS) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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