ATG5 Knockout cell line (NCI-H1299)

location: Home > Products > KO Cell Lines > ATG5 Knockout cell line (NCI-H1299)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

ATG5 Knockout cell line (NCI-H1299) Order

ATG5 Knockout cell line (NCI-H1299)
Catalog#
YKO-H238
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ATG5 Knockout cell line (NCI-H1299)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ATG5 knockout cell line (NCI-H1299)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
ATG5
Gene ID
9474
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Target region
Knockout region: Exon 2 (based on transcript ATG5-204)
Validation: PCR+Sanger Sequencing
ATG5 Knockout cell line (NCI-H1299) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H1299
STR Info (Cell bank)
Cell Line:NCI-H1299-Luc2-tdT-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 12121212
D7S820 10101010
D16S539 12131213
VWA 16181618
TH01 69.369.3
AMEL XXXX
TPOX 8888
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ATG5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NCI-H1299 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ATG5 gene knockout ATG5 knockout cell line (NCI-H1299) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

  • NCI-H1299 cell line

    Human Non-small Cell Lung Cancer Cell Line(NCI-H1299)

    Catalog#: YC-D010
    Size: 1*10^6
    Instruction: Human Non-small Cell Lung Cancer Cell Line(NCI-H1299)

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • Atg5 Knockout cell line  (HT22)

    Atg5 Knockout cell line (HT22)

    Organism: Mouse
    Gene Name: Atg5
    Gene ID: 11793
    Size: 1*10^6
    Catalog#: YKO-M197
    YKO-M197(HT22)
  • ATG5 Knockout cell line (HCT 116)

    ATG5 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ATG5
    Gene ID: 9474
    Size: 1*10^6
    Catalog#: YKO-XN85677
    YKO-XN85677(HCT 116)
  • ATG5 Knockout cell line (HEK293)

    ATG5 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: ATG5
    Gene ID: 9474
    Size: 1*10^6
    Catalog#: YKO-XN85676
    YKO-XN85676(HEK293)

Related Popular Products

Wild-type Cell Lines

  • NCI-H1299 cell line

    Human Non-small Cell Lung Cancer Cell Line(NCI-H1299)

    Price (USD): -
    Catalog#: YC-D010
    Size: 1*10^6
    Instruction: Human Non-small Cell Lung Cancer Cell Line(NCI-H1299)

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • Atg5 Knockout cell line  (HT22)

    Atg5 Knockout cell line (HT22)

    Organism: Mouse
    Gene Name: Atg5
    Gene ID: 11793
    Size: 1*10^6
    Catalog#: YKO-M197
  • ATG5 Knockout cell line (HCT 116)

    ATG5 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ATG5
    Gene ID: 9474
    Size: 1*10^6
    Catalog#: YKO-XN85677
  • ATG5 Knockout cell line (HEK293)

    ATG5 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: ATG5
    Gene ID: 9474
    Size: 1*10^6
    Catalog#: YKO-XN85676

Contact us