BTK Knockout cell line(U-2932)

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BTK Knockout cell line(U-2932) Order

BTK Knockout cell line(U-2932)
Catalog#
YKO-H067
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
BTK Knockout cell line(U-2932)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
BTK Knockout cell line (U-2932)
Morphology
Human Lymphocyte-like, suspension
Passage ratio
Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml.
Species
Human
Gene Name
BTK
Gene ID
695
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 2 (based on transcript BTK-201)
Validation: PCR+Sanger Sequencing
BTK Knockout cell line(U-2932) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:U-2932
STR Info (Cell bank)
Cell Line:U-2932
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11111111
D7S820 913913
D16S539 12121212
VWA 14171417
TH01 9.39.39.39.3
AMEL XXXX
TPOX 11121112
CSF1PO 10121012
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BTK gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-2932 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BTK gene knockout BTK Knockout cell line (U-2932) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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