CDR1 Knockout cell line(OVCAR-3)

location: Home > Products > KO Cell Lines > CDR1 Knockout cell line(OVCAR-3)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

CDR1 Knockout cell line(OVCAR-3) Order

CDR1 Knockout cell line(OVCAR-3)
Catalog#
YKO-H123
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CDR1 Knockout cell line(OVCAR-3)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CDR1 Knockout cell line (OVCAR-3)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
CDR1
Gene ID
1038
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
80%RPMI-1640+20% FBS+0.01mg/mL Insulin
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:OVCAR-3
STR Info (Cell bank)
Cell Line:OVCAR-3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 12121212
D7S820 10101010
D16S539 12121212
VWA 17171717
TH01 9999
AMEL XXXX
TPOX 8888
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CDR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the OVCAR-3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CDR1 gene knockout CDR1 Knockout cell line (OVCAR-3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

  • OVCAR-3 cell line

    Human Ovarian Cancer Cell Line(OVCAR-3)

    Catalog#: YC-D019
    Size: 1*10^6
    Instruction: Human Ovarian Cancer Cell Line(OVCAR-3)

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

Contact us