CDR2L Knockout cell line(OVCAR-3)

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CDR2L Knockout cell line(OVCAR-3) Order

CDR2L Knockout cell line(OVCAR-3)
Catalog#
YKO-H096
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CDR2L Knockout cell line(OVCAR-3)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CDR2L Knockout cell line (OVCAR-3)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
CDR2L
Gene ID
30850
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
80%RPMI-1640+20% FBS+0.01mg/mL Insulin
Target region
Knockout region: Exon 2 (based on transcript CDR2L-201)
Validation: PCR+Sanger Sequencing
CDR2L Knockout cell line(OVCAR-3) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:OVCAR-3
STR Info (Cell bank)
Cell Line:OVCAR-3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 12121212
D7S820 10101010
D16S539 12121212
VWA 17171717
TH01 9999
AMEL XXXX
TPOX 8888
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CDR2L gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the OVCAR-3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CDR2L gene knockout CDR2L Knockout cell line (OVCAR-3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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