CDYL Knockout cell line (H9)

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CDYL Knockout cell line (H9) Order

CDYL Knockout cell line (H9)
Catalog#
YKO-H282
Size
2*10^5
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CDYL Knockout cell line (H9)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CDYL knockout cell line (H9)
Morphology
Spherical clone
Passage ratio
1:6-1:8
Species
Human
Gene Name
CDYL
Gene ID
9425
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
Target region
Knockout region: Exon 2 (based on transcript CDYL-203)
Validation: PCR+Sanger Sequencing
CDYL Knockout cell line (H9) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:H9
STR Info (Cell bank)
Cell Line:WAe009-A-5
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 9999
D7S820 911911
D16S539 12131213
VWA 17171717
TH01 9.39.39.39.3
AMEL XXXX
TPOX 10111011
CSF1PO 11111111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CDYL gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H9 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CDYL gene knockout CDYL knockout cell line (H9) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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