DGKZ Knockout cell line(Jurkat)

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DGKZ Knockout cell line(Jurkat) Order

DGKZ Knockout cell line(Jurkat)
Catalog#
YKO-H203
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
DGKZ Knockout cell line(Jurkat)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
DGKZ Knockout cell line (Jurkat)
Morphology
Lymphocyte-like,suspension
Passage ratio
Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml.
Species
Human
Gene Name
DGKZ
Gene ID
8525
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 2 (based on transcript DGKZ-205)
Validation: PCR+Sanger Sequencing
DGKZ Knockout cell line(Jurkat) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:Jurkat
STR Info (Cell bank)
Cell Line:Jurkat, Clone E6-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 9999
D13S317 812812
D7S820 812812
D16S539 11111111
VWA 18181818
TH01 69.369.3
AMEL XYXY
TPOX 810810
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the DGKZ gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, DGKZ gene knockout DGKZ Knockout cell line (Jurkat) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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