DSC2 Knockout cell line(MGC80-3)

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DSC2 Knockout cell line(MGC80-3) (Hela-contaminated cells, not for sales) Order

DSC2 Knockout cell line(MGC80-3)
Catalog#
YKO-H037
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
DSC2 Knockout cell line(MGC80-3)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
DSC2 Knockout cell line (MGC80-3)
Morphology
Epithelial-like,adherent
Passage ratio
1:3-1:4
Species
Human
Gene Name
DSC2
Gene ID
1824
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:MGC80-3
STR Info (Cell bank)
Cell Line:MGC-803
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
D5S818 101112.0101112.0
D13S317 713.3713.3
D7S820 11121112
D16S539 911911
VWA 161718.0161718.0
TH01 7979
AMEL XXXX
TPOX 12121212
CSF1PO 91012.091012.0
* Hela-contaminated cells, not for sales
Reviewed by ICLAC (ref: 191110). MGC-803 was reported to originally come from gastric carcinoma but several papers from cell repositories in China have shown that it has a high similarity to HeLa. Looking at the STR profiles that we have available, they appear to fall into two groups. One is consistent with HeLa, while the other has additional alleles at many loci e.g. Penta E has "7,10,16,17". This second group of samples may represent a mixture of HeLa and a second cell line; it may also represent a hybrid cell line (e.g., following exposure to a fusogenic agent).

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the DSC2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MGC80-3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, DSC2 gene knockout DSC2 Knockout cell line (MGC80-3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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