DUS3L Knockout cell line(786-O)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > DUS3L Knockout cell line(786-O)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

DUS3L Knockout cell line(786-O) Order

DUS3L Knockout cell line(786-O)
Catalog#
YKO-H304
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
DUS3L Knockout cell line(786-O)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
DUS3L Knockout cell line (786-O)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:4
Species
Human
Gene Name
DUS3L
Gene ID
56931
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_D7IX(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript DUS3L-201)
Validation: PCR+Sanger Sequencing
DUS3L Knockout cell line(786-O) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:786-O
STR Info (Cell bank)
Cell Line:786-O [786-0]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 9999
D13S317 8888
D7S820 11121112
D16S539 12121212
VWA 15171517
TH01 69.369.3
AMEL XYXY
TPOX 811811
CSF1PO 10101010
D12S391 1718
FGA 2424
D2S1338 1718
D21S11 2930
D18S51 1314
D8S1179 1313
D3S1358 1616
D6S1043 1111
PENTAE 716
D19S433 1415
PENTAD 912
D1S1656 1112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the DUS3L gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 786-O cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, DUS3L gene knockout DUS3L Knockout cell line (786-O) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
DUS3L Knockout cell line(786-O)
DUS3L Knockout cell line(786-O)
Catalog#
YKO-H304
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • DUS3L Knockout cell line (HCT 116)

    DUS3L Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87731
    YKO-XN87731(HCT 116)
  • DUS3L Knockout cell line (HEK293)

    DUS3L Knockout cell line (HEK293)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87730
    YKO-XN87730(HEK293)
  • DUS3L Knockout cell line (A549)

    DUS3L Knockout cell line (A549)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87729
    YKO-XN87729(A549)

Related Popular Products

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • DUS3L Knockout cell line (HCT 116)

    DUS3L Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87731
  • DUS3L Knockout cell line (HEK293)

    DUS3L Knockout cell line (HEK293)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87730
  • DUS3L Knockout cell line (A549)

    DUS3L Knockout cell line (A549)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87729

Contact us