DUS3L Knockout cell line(786-O)

location: Home > Products > KO Cell Lines > DUS3L Knockout cell line(786-O)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

DUS3L Knockout cell line(786-O) Order

DUS3L Knockout cell line(786-O)
Catalog#
YKO-H304
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
DUS3L Knockout cell line(786-O)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
DUS3L Knockout cell line (786-O)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:4
Species
Human
Gene Name
DUS3L
Gene ID
56931
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 2 (based on transcript DUS3L-201)
Validation: PCR+Sanger Sequencing
DUS3L Knockout cell line(786-O) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:786-O
STR Info (Cell bank)
Cell Line:786-O [786-0]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 9999
D13S317 8888
D7S820 11121112
D16S539 12121212
VWA 15171517
TH01 69.369.3
AMEL XYXY
TPOX 811811
CSF1PO 10101010
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the DUS3L gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 786-O cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, DUS3L gene knockout DUS3L Knockout cell line (786-O) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • DUS3L Knockout cell line (HCT 116)

    DUS3L Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87731
    YKO-XN87731(HCT 116)
  • DUS3L Knockout cell line (HEK293)

    DUS3L Knockout cell line (HEK293)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87730
    YKO-XN87730(HEK293)
  • DUS3L Knockout cell line (A549)

    DUS3L Knockout cell line (A549)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87729
    YKO-XN87729(A549)

Related Popular Products

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • DUS3L Knockout cell line (HCT 116)

    DUS3L Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87731
  • DUS3L Knockout cell line (HEK293)

    DUS3L Knockout cell line (HEK293)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87730
  • DUS3L Knockout cell line (A549)

    DUS3L Knockout cell line (A549)

    Organism: Human
    Gene Name: DUS3L
    Gene ID: 56931
    Size: 1*10^6
    Catalog#: YKO-XN87729

Contact us