DVL3 Knockout cell line(HeLa)

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DVL3 Knockout cell line(HeLa) Order

DVL3 Knockout cell line(HeLa)
Catalog#
YKO-H313
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
DVL3 Knockout cell line(HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
DVL3 Knockout cell line (HeLa)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
DVL3
Gene ID
1857
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 2 (based on transcript DVL3-201)
Validation: PCR+Sanger Sequencing
DVL3 Knockout cell line(HeLa) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the DVL3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, DVL3 gene knockout DVL3 Knockout cell line (HeLa) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
DVL3 Knockout cell line(HeLa)
DVL3 Knockout cell line(HeLa)
Catalog#
YKO-H313
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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KO Cell Lines

  • DVL3 Knockout cell line (HCT 116)

    DVL3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: DVL3
    Gene ID: 1857
    Size: 1*10^6
    Catalog#: YKO-HS2274
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    Gene Name: DVL3
    Gene ID: 1857
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    Organism: Human
    Gene Name: DVL3
    Gene ID: 1857
    Size: 1*10^6
    Catalog#: YKO-HS026

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