EGR1 Knockout cell line(THP-1)

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EGR1 Knockout cell line(THP-1) Order

EGR1 Knockout cell line(THP-1)
Catalog#
YKO-H127
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
EGR1 Knockout cell line(THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
EGR1 Knockout cell line (THP-1)
Morphology
Monocyte,suspension
Passage ratio
Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml.
Species
Human
Gene Name
EGR1
Gene ID
1958
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 1 (based on transcript EGR1-201)
Validation: PCR+Sanger Sequencing
EGR1 Knockout cell line(THP-1) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:THP-1
STR Info (Cell bank)
Cell Line:THP-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 13131313
D7S820 10101010
D16S539 11121112
VWA 16161616
TH01 89.389.3
AMEL XYXY
TPOX 811811
CSF1PO 11131113
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the EGR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, EGR1 gene knockout EGR1 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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