EREG Knockout cell line(U-87 MG)

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EREG Knockout cell line(U-87 MG) Order

EREG Knockout cell line(U-87 MG)
Catalog#
YKO-H092
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
EREG Knockout cell line(U-87 MG)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
EREG Knockout cell line (U-87 MG)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
EREG
Gene ID
2069
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 2 (based on transcript EREG-201)
Validation: PCR+Sanger Sequencing
EREG Knockout cell line(U-87 MG) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:U-87 MG
STR Info (Cell bank)
Cell Line:U-87MG
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 811811
D7S820 8989
D16S539 12121212
VWA 15171517
TH01 9.39.39.39.3
AMEL XXXX
TPOX 8888
CSF1PO 10111011
D12S391 1821
FGA 1824
D2S1338 2023
D21S11 2832.2
D18S51 1313
D8S1179 1011
D3S1358 1617
D6S1043 1118
PENTAE 714
D19S433 1515.2
PENTAD 914
D1S1656 1515
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the EREG gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-87 MG cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, EREG gene knockout EREG Knockout cell line (U-87 MG) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
EREG Knockout cell line(U-87 MG)
EREG Knockout cell line(U-87 MG)
Catalog#
YKO-H092
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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