ERVFRD-1 Knockout cell line(BeWo)

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ERVFRD-1 Knockout cell line(BeWo) Order

ERVFRD-1 Knockout cell line(BeWo)
Catalog#
YKO-H208
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ERVFRD-1 Knockout cell line(BeWo)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ERVFRD-1 Knockout cell line (BeWo)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
ERVFRD-1
Gene ID
405754
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
50%DMEM/F12+40%FBS+10%DMSO
Target region
Knockout region: Exon 2 (based on transcript ERVFRD-1-201)
Validation: PCR+Sanger Sequencing
ERVFRD-1 Knockout cell line(BeWo) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:BeWo
STR Info (Cell bank)
Cell Line:BEWO
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 911911
D7S820 10121012
D16S539 13141314
VWA 16161616
TH01 99.399.3
AMEL XYXY
TPOX 8888
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ERVFRD-1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BeWo cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ERVFRD-1 gene knockout ERVFRD-1 Knockout cell line (BeWo) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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