ESRP2 Knockout cell line(HT-29)

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location： Home > Products > KO Cell Lines > ESRP2 Knockout cell line(HT-29)

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ESRP2 Knockout cell line(HT-29) Order

ESRP2 Knockout cell line(HT-29)
Catalog#
YKO-H187
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ESRP2 Knockout cell line(HT-29)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ESRP2 Knockout cell line (HT-29)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
ESRP2
Gene ID
80004
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Target region
Knockout region: Exon 3 (based on transcript ESRP2-202)
Validation: PCR+Sanger Sequencing
ESRP2 Knockout cell line(HT-29) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HT-29		 STR Info (Cell bank)
Cell Line:HT-29
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11121112
D7S820 10101010
D16S539 11121112
VWA 17191719
TH01 6969
AMEL XXXX
TPOX 8989
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ESRP2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT-29 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ESRP2 gene knockout ESRP2 Knockout cell line (HT-29) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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