FGFR1 Knockout cell line(HGC-27)

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FGFR1 Knockout cell line(HGC-27) Order

FGFR1 Knockout cell line(HGC-27)
Catalog#
YKO-H209
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
FGFR1 Knockout cell line(HGC-27)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
FGFR1 Knockout cell line (HGC-27)
Morphology
Endothelial cells, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
FGFR1
Gene ID
2260
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Target region
Knockout region: Exon 4,Exon 5 (based on transcript FGFR1-214)
Validation: PCR+Sanger Sequencing
FGFR1 Knockout cell line(HGC-27) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HGC-27
STR Info (Cell bank)
Cell Line:HGC-27
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 10111011
D7S820 111213.0111213.0
D16S539 10111011
VWA 14141414
TH01 9999
AMEL XXXX
TPOX 8888
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FGFR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HGC-27 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FGFR1 gene knockout FGFR1 Knockout cell line (HGC-27) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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