FKBP10 Knockout cell line(HT-29)

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FKBP10 Knockout cell line(HT-29) Order

FKBP10 Knockout cell line(HT-29)
Catalog#
YKO-H210
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
FKBP10 Knockout cell line(HT-29)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
FKBP10 Knockout cell line (HT-29)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
FKBP10
Gene ID
60681
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Target region
Knockout region: Exon 2 (based on transcript FKBP10-201)
Validation: PCR+Sanger Sequencing
FKBP10 Knockout cell line(HT-29) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HT-29
STR Info (Cell bank)
Cell Line:HT-29
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11121112
D7S820 10101010
D16S539 11121112
VWA 17191719
TH01 6969
AMEL XXXX
TPOX 8989
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FKBP10 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT-29 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FKBP10 gene knockout FKBP10 Knockout cell line (HT-29) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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