GSDMB Knockout cell line (HTR-8/SVneo)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > GSDMB Knockout cell line (HTR-8/SVneo)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

GSDMB Knockout cell line (HTR-8/SVneo) Order

GSDMB Knockout cell line (HTR-8/SVneo)
Catalog#
YKO-H233
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GSDMB Knockout cell line (HTR-8/SVneo)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GSDMB knockout cell line (HTR-8/SVneo)
Morphology
Endothelial cells, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
GSDMB
Gene ID
55876
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript GSDMB-205)
Validation: PCR+Sanger Sequencing
GSDMB Knockout cell line (HTR-8/SVneo) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:HTR-8/SVneo
STR Info (Cell bank)
Cell Line:HTR-8/SVneo
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 912912
D7S820 12121212
D16S539 11131313
VWA 13181318
TH01 69.369.3
AMEL XXXX
TPOX 8888
CSF1PO 12121212
D12S391 1919
FGA 2123
D2S1338 2324
D21S11 2930
D18S51 1313
D8S1179 1515
D3S1358 1616
D6S1043 1419
PENTAE 77
D19S433 1415
PENTAD 912
D1S1656 1617.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GSDMB gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HTR-8/SVneo cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GSDMB gene knockout GSDMB knockout cell line (HTR-8/SVneo) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GSDMB Knockout cell line (HTR-8/SVneo)
GSDMB Knockout cell line (HTR-8/SVneo)
Catalog#
YKO-H233
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • GSDMB Knockout cell line (HeLa)

    GSDMB Knockout cell line (HeLa)

    Organism: Human
    Gene Name: GSDMB
    Gene ID: 55876
    Size: 1*10^6
    Catalog#: YKO-XN89041
    YKO-XN89041(HeLa)
  • GSDMB Knockout cell line (HCT 116)

    GSDMB Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: GSDMB
    Gene ID: 55876
    Size: 1*10^6
    Catalog#: YKO-XN89040
    YKO-XN89040(HCT 116)
  • GSDMB Knockout cell line (HEK293)

    GSDMB Knockout cell line (HEK293)

    Organism: Human
    Gene Name: GSDMB
    Gene ID: 55876
    Size: 1*10^6
    Catalog#: YKO-XN89039
    YKO-XN89039(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • GSDMB Knockout cell line (HeLa)

    GSDMB Knockout cell line (HeLa)

    Organism: Human
    Gene Name: GSDMB
    Gene ID: 55876
    Size: 1*10^6
    Catalog#: YKO-XN89041
  • GSDMB Knockout cell line (HCT 116)

    GSDMB Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: GSDMB
    Gene ID: 55876
    Size: 1*10^6
    Catalog#: YKO-XN89040
  • GSDMB Knockout cell line (HEK293)

    GSDMB Knockout cell line (HEK293)

    Organism: Human
    Gene Name: GSDMB
    Gene ID: 55876
    Size: 1*10^6
    Catalog#: YKO-XN89039

Contact us