IGF2BP3 Knockout cell line(ACHN)

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location： Home > Products > KO Cell Lines > IGF2BP3 Knockout cell line(ACHN)

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IGF2BP3 Knockout cell line(ACHN) Order

IGF2BP3 Knockout cell line(ACHN)
Catalog#
YKO-H317
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
IGF2BP3 Knockout cell line(ACHN)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
IGF2BP3 Knockout cell line (ACHN)
Morphology
Epithelial-like,adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
IGF2BP3
Gene ID
10643
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 1 (based on transcript IGF2BP3-201)
Validation: PCR+Sanger Sequencing
IGF2BP3 Knockout cell line(ACHN) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:ACHN		 STR Info (Cell bank)
Cell Line:ACHN
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 12121212
D7S820 911911
D16S539 12131213
VWA 16171617
TH01 8888
AMEL XXXX
TPOX 811811
CSF1PO 11111111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the IGF2BP3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the ACHN cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, IGF2BP3 gene knockout IGF2BP3 Knockout cell line (ACHN) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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