KRAS Knockout cell line(RKO)

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KRAS Knockout cell line(RKO) Order

KRAS Knockout cell line(RKO)
Catalog#
YKO-H251
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
KRAS Knockout cell line(RKO)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
KRAS Knockout cell line (RKO)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
KRAS
Gene ID
3845
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript KRAS-202)
Validation: PCR+Sanger Sequencing
KRAS Knockout cell line(RKO) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:RKO
STR Info (Cell bank)
Cell Line:RKO
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 811811
D7S820 810810
D16S539 13131213
VWA 16221622
TH01 610610
AMEL XXXX
TPOX 11111111
CSF1PO 810810
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KRAS gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RKO cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KRAS gene knockout KRAS Knockout cell line (RKO) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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