KREMEN1 Knockout cell line(NCI-H1299)

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KREMEN1 Knockout cell line(NCI-H1299) Order

KREMEN1 Knockout cell line(NCI-H1299)
Catalog#
YKO-H011
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
KREMEN1 Knockout cell line(NCI-H1299)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
KREMEN1 Knockout cell line (NCI-H1299)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
KREMEN1
Gene ID
83999
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Target region
Knockout region: Exon 2 (based on transcript KREMEN1-202)
Validation: PCR+Sanger Sequencing
KREMEN1 Knockout cell line(NCI-H1299) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:NCI-H1299
STR Info (Cell bank)
Cell Line:NCI-H1299-Luc2-tdT-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 12121212
D7S820 10101010
D16S539 12131213
VWA 16181618
TH01 69.369.3
AMEL XXXX
TPOX 8888
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KREMEN1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NCI-H1299 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KREMEN1 gene knockout KREMEN1 Knockout cell line (NCI-H1299) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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