LGALS3 Knockout cell line(JIMT-1)

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LGALS3 Knockout cell line(JIMT-1) Order

LGALS3 Knockout cell line(JIMT-1)
Catalog#
YKO-H121
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LGALS3 Knockout cell line(JIMT-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LGALS3 Knockout cell line (JIMT-1)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:5
Species
Human
Gene Name
LGALS3
Gene ID
3958
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%DMEM+12%FBS
Target region
Knockout region: Exon 3 (based on transcript LGALS3-201)
Validation: PCR+Sanger Sequencing
LGALS3 Knockout cell line(JIMT-1) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:JIMT-1
STR Info (Cell bank)
Cell Line:JIMT-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 121314.0121314.0
D13S317 813813
D7S820 12121212
D16S539 11111111
VWA 18181818
TH01 7777
AMEL XXXX
TPOX 8888
CSF1PO 12131213
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LGALS3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the JIMT-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LGALS3 gene knockout LGALS3 Knockout cell line (JIMT-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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