LIPA Knockout cell line (SH-SY5Y)

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LIPA Knockout cell line (SH-SY5Y) Order

LIPA Knockout cell line (SH-SY5Y)
Catalog#
YKO-H261
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LIPA Knockout cell line (SH-SY5Y)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LIPA knockout cell line (SH-SY5Y)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Gene Name
LIPA
Gene ID
3988
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 4 (based on transcript LIPA-202)
Validation: PCR+Sanger Sequencing
LIPA Knockout cell line (SH-SY5Y) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:SH-SY5Y
STR Info (Cell bank)
Cell Line:SH-SY5Y
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11111111
D7S820 710710
D16S539 813813
VWA 14181418
TH01 710710
AMEL XXXX
TPOX 811811
CSF1PO 11111111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LIPA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SH-SY5Y cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LIPA gene knockout LIPA knockout cell line (SH-SY5Y) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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