MAGEA4 Knockout cell line(A-375)

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MAGEA4 Knockout cell line(A-375) Order

MAGEA4 Knockout cell line(A-375)
Catalog#
YKO-H274
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MAGEA4 Knockout cell line(A-375)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MAGEA4 Knockout cell line (A-375)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
MAGEA4
Gene ID
4103
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 3 (based on transcript MAGEA4-201)
Validation: PCR+Sanger Sequencing
MAGEA4 Knockout cell line(A-375) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:A-375
STR Info (Cell bank)
Cell Line:A-375 [A375]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11141114
D7S820 9999
D16S539 9999
VWA 16171617
TH01 8888
AMEL XXXX
TPOX 810810
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MAGEA4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A-375 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MAGEA4 gene knockout MAGEA4 Knockout cell line (A-375) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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