NCOA3 Knockout cell line(MCF7)

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NCOA3 Knockout cell line(MCF7) Order

NCOA3 Knockout cell line(MCF7)
Catalog#
YKO-H159
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NCOA3 Knockout cell line(MCF7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NCOA3 Knockout cell line (MCF7)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
NCOA3
Gene ID
8202
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
87%MEM+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA
Target region
Knockout region: Exon 4 (based on transcript NCOA3-202)
Validation: PCR+Sanger Sequencing
NCOA3 Knockout cell line(MCF7) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:MCF7
STR Info (Cell bank)
Cell Line:MCF-7
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11111111
D7S820 8989
D16S539 11121112
VWA 14151415
TH01 6666
AMEL XXXX
TPOX 912912
CSF1PO 10101010
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NCOA3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MCF7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NCOA3 gene knockout NCOA3 Knockout cell line (MCF7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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