By subscription, you consent to allow Ubigene Biosciences to store and process the information
provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe
from these communications at anytime.
Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation
CRISPR-U™ technology. At the same time, we already provide customers with high quality gene-editing
tools for cell or animal research worldwide.
With 14 years of experience, Ubigene has exclusively innovated and developed 6 product lines,
fullfilling all kinds of needs from researchers. Experiment process simplified, efficiency improved,
achieving our aim of 'Make genome editing easier'!
ExPASy Cellosaurus Database Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy
for the PACC1 gene, clone the two sgRNAs into CRISPR-U™ vector,
transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution
method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic
acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype.
Upon passing the verification, PACC1 gene knockout PACC1 Knockout cell line (HEK293) will be expanded and cryopreserved.